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TWENTY-FOURTH ANNUAL EASTERN FISH HEALTH WORKSHOP


Royal Pavilion Resort, Atlantic Beach, NC
MARCH 9-11, 1999


Isolation And Identification Of Viruses From Adult Largemouth Bass During A 1997–1998 Survey In The Southeastern United States

John A. Plumb1, Andrew D. Noyes1, Sara Graziano1, Jun Wang2, Jinghe Mao2, and V. Gregory Chinchar2

1Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849; 2Department of Microbiology, University of Mississippi Medical Center, Jackson,MS 39216

Largemouth bass Micropterus salmoides were electrofished from 85 sites (reservoirs, lakes or rivers) in eight southeastern states and screened for the presence of largemouth bass virus (LMBV) from August 1997 to January 1999. From each location, between 3 and 10 fish were sampled and 474 bass were used in the entire study. Virus was isolated from bass from seven different reservoirs on five different river systems in Alabama and Georgia. These bodies of water were Lake Jordan and Allatoona (Coosa River), Lake Wilson (Tennessee River), Lake Walter F. George and Lake Oliver (Chattahoochee River), Lake Blackshear (Flint River) and Lake Greenwood (Santee). No virus was isolated from bass from 74 other sites in Alabama, Florida, Georgia, Kentucky, Louisiana, Missouri, South Carolina and Virginia. Viral isolates from four sites (Lakes Walter F. George, Jordan, Blackshear and Allatoona) had DNA sequences from a major capsid gene which were identical to the original LMBV isolate from the Santee–Cooper Reservoir System.

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