Acute Causes Of Sea Turtle Death:
Identifying Turtle In Your Soup
M. Katherine Moore1, John
A. Bemiss1, Susan M. Rice2, Joseph M. Quattro3,
and Cheryl M. Woodley1
1National Oceanic
and Atmospheric Administration, National Ocean Service, National Centers for
Coastal Ocean Science, Center for Coastal Environmental Health and Biomolecular
Research at Charleston, Charleston, SC; 2U. S. Fish and Wildlife
Service, Eastern Shore of Virginia National Wildlife Refuge, Cape Charles, VA; 3Department
of Biological Sciences, Baruch Institute, School of the Environment, Program in
Marine Science, University of South Carolina, Columbia, SC
One
of the many threats to sea turtle populations is poaching of turtles and their
eggs for consumption and sale. Here, we will address the scope of the illegal
trade and introduce a new method to identify sea turtle eggs and cooked meats
to the species level. Currently, fatty acid profiles that are used to identify
eggs to species cannot resolve differences between loggerheads and the two
ridley species. Protein-based methods can resolve all species, but can only be
used for fresh meat, not eggs or cooked (or otherwise degraded) meat. In this
study, we present methods to produce species-specific diagnostic restriction
fragment length polymorphism patterns in the cytochrome B region of the
mitochondrial DNA. This method will work on DNA of sufficient quality,
regardless of the tissue of origin, and provides wildlife law enforcement
another tool to combat illegal take of endangered species.
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