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Recent
Developments In The Spread And Study Of Marine Turtle Fibropapillomatosis And
The Fibropapilloma Associated Turtle Herpesvirus
Rebecca J. Murcek1, Claudia
A. Sutton1, Sandra L. Quackenbush2, Thierry M. Work3,
Rufina N. Casey1, Joel Rovnak2, George H. Balazs4,
James W. Casey1
1Cornell
University, Dept of Microbiology and Immunology, Ithaca NY; 2University
of Kansas, Dept of Molecular Biosciences, Lawrence, KS; 3USGS,
National Wildlife Health Center Honolulu Field Station, HI; 4National
Marine Fisheries Service, Honolulu Laboratory, HI
Fibropapillomatosis (FP) of marine turtles is a recently emerging
disease that affects turtles worldwide. Polymerase Chain Reaction (PCR) studies
identified the polymerase gene of a herpesvirus, named the Fibropapilloma
Associated Turtle Herpesvirus (FPTHV), in tumor samples taken from FP-affected
turtles. Quantitative PCR (qPCR) has demonstrated that FPTHV is present at high
copy numbers (1 to 50 copies per cell) in FP tumor tissue. We examined the
tissues of five different types of parasites (marine leeches, amphipods, three
species of blood flukes, Pyelosomum
longicaecum bladder parasites, and barnacles) of FP-affected Hawaiian green
turtles (Chelonia mydas) to determine
whether any of them carried enough virus to be possible vector organisms for
FPTHV. Leeches were found to frequently carry high levels of viral DNA (10^6 to
10^7 copies per parasite organism). We have also found detectable levels of
FPTHV in seawater from Kaneohe Bay. Most recently, we have cloned and sequenced
approximately 30 kb of the FPTHV genome, confirming its classification as an
alphaherpesvirus.
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