Infectious Salmon Anaemia Is Not Vertically Transmitted; Evidence From Recent Experiments In New Brunswick, Canada     Gilles Olivier1, Anne-Margaret MacKinnon1, Steve G. Griffiths2   1Department of Fisheries and Oceans, Science Branch,  Gulf Fisheries Centre, Gulf Region, Moncton, New Brunswick, Canada; 2Research and Productivity Council, 921 College Hill Road, Fredericton, New Brunswick, Canada      Melville and Griffiths (1999) have provided evidence that Infectious Salmon Anaemia (ISA) virus is not vertically transferred from individually infected Atlantic salmon. In the fall of 1999, 15 wild adult Atlantic salmon held in a land-based facility experienced an ISA outbreak. The ISA virus was recovered from blood, tissues and ovarian fluids.  Eggs from infected Salmon were separated into two groups, a disinfected lot and a non-disinfected lot.  The progeny of both groups were kept in quarantine and tested at regular intervals for the presence of ISAv by IFAT, cell culture and RT-PCR.  To this date, fish are alive and held in quarantine, ISA was never observed in these fish and no ISA virus was isolated from any fish tested by cell culture. The only positive results were those obtained by RT-PCR several times during their regular testing regime. The interpretation and significance of these results will be discussed.