<TWENTY-THIRD ANNUAL EASTERN FISH HEALTH WORKSHOP JOHN CARVER INN, PLYMOUTH, MA 30 MARCH - 2 APRIL, 1998 Infectious Pancreatic Necrosis Virus: Transmission From Infectious To Susceptible Rainbow Trout Fry Julie Bebak1, P. E. McAllister2 and G. Smith3 1Freshwater Institute, Post Office Box 1746, Shepherdstown, WV 25443; 2U.S. Geological Survey, Biological Resources Division, National Fish Health Research Laboratory, Leetown Science Center, 1700 Leetown Road, Kearneysville, WV 25430; 3Center for Infectious Disease and Food Safety, University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348 Rainbow trout (Oncorhynchus mykiss) fry were exposed to serotype VR-299 of infectious pancreatic necrosis virus (IPNV) using a standardized immersion challenge. In concurrent experiments, fish were monitored for 11 d for excretion of IPNV, or monitored for 9 d for excretion and transmission of IPNV to susceptible rainbow trout fry. Immersion-challenged fish began excreting virus within 2 d after challenge. The rate of IPNV excretion per fish increased steadily from about day four to day eight, and then decreased. Virus concentrations in tissues of immersion-challenged fish increased exponentially. Susceptible fish became infected with IPNV within 4 d after being introduced to immersion-challenged fish (e.g., 2 d after the challenged fish began excreting virus). By 9 d 84% of the susceptible fish were infected with IPNV.   Return to 23rd Annual Eastern Fish Health Workshop Return to Leetown Science Center Home Page